Sample material: Plasma, serum or aqueous solutions.
Measurement range: 0-30.0 g/L (0-3.00 g/dL, 0-3000 mg/dL, 0-1.90 mmol/L).
Results: In about 60 seconds.
Sample volume: 20 µL.
Weight: 1 kg (2 pounds) with batteries installed.
Power: AC Adapter or batteries.
Operating temperature: 15-40 °C (59-104 °F).
Quality control: Two levels of liquid control is recommended to be run on the day of use, or as required by accrediting agencies or local policy.
Calibration: The photometer is calibrated at the factory against the ICSH reference method and needs no further calibration.
Storage for HemoCue® Plasma/Low Hb Photometer: The photometer can be stored at temperature 0-50 °C (32-122 °F).
Storage for HemoCue® Plasma/Low Hb Microcuvettes: The microcuvettes are to be stored at 15-30 °C (59-86 °F). Once the vial is opened the microcuvettes are stable for three months.
Replace the cap immediately after microcuvettes are removed from the vial.
Method: Vanzetti, G. An azidemethemoglobin method for hemoglobin determination in blood. J. Lab. Clin. Med. 67, 116-26 (1966).
Principle: The reaction in the microcuvette is a modified azidemethemoglobin reaction. The erythrocyte membranes are disintegrated by sodium deoxycholate, releasing the hemoglobin. Sodium nitrite converts the hemoglobin iron from the ferrous to the ferric state to form methemoglobin, which then combines with azide to form azidmethemoglobin. The photometer uses a double wavelength measuring method, 570 nm and 880 nm, for compensation for a certain degree of turbidity.